Open date: September 14th, 2020
Last review date: Thursday, Dec 31, 2020 at 11:59pm (Pacific Time)
Applications received after this date will be reviewed by the search committee if the position has not yet been filled.
Final date: Wednesday, Jun 30, 2021 at 11:59pm (Pacific Time)
Applications will continue to be accepted until this date, but those received after the review date will only be considered if the position has not yet been filled.
The Division of Hematology-Oncology in the Department of Pediatrics of the David Geffen School of Medicine at UCLA is seeking a Project Scientist. The appointment will be in the Project Scientist series; academic rank will be commensurate with experience.
This position requires a Ph.D. or equivalent and prior experience. This is a Non-Senate position.
- (Manufacturing products for clinical research according to Good Manufacturing Practice (GMP) guidelines
- Operate in a GMP regulated environment according current standard operating protocols
- Meticulous record keeping and maintenance of GMP, GTP and basic research related records and documents.
- Attend and arrange seminars, training sessions and workshops to maintain competency in evolving current GMP/GTP regulations
- Performing post-manufacturing lot-release tests: Elisa, Colony-forming Unit (CFU) assay, ELISA, Endotoxin assay, Mycoplasma assay, vector copy number (VCN) test by ddPCR
- Culture of hematopoietic progenitor cells for in vitro differentiation into T-cells for proof of principle testing of gene delivery
- Conduct experiments for FDA IND application(s)
- Conduct DNA, RNA and protein isolation and purification according current GLP research grade protocols
- Quantification of nucleic acids and proteins
- Perform PCR and qPCR
- Perform multiparametric flow cytometry to assist on-going specific NYESO-TCR research project for intracellular, surface and peptide target markers.
- Culture adhered and non-adherent mammalian cells, preparing media, aliquots and other compounds utilized in tissue culture with proper sterile aseptic technique.
- Ability to set up co-cultures for killing-assays or peptide release production-analysis
- Ability to set up experiments utilizing real time in vitro cell imaging system Incucyte
- Perform ELISA experiments in either cellular supernatants or cellular lysates normalizing results against total protein content
- Plasmid preparation, bacteria growth on competent E. coli or Stable 3 systems, positive transformants selection and sequences analysis
- Ability of align sequences on APE-software (or-equivalent) and design plasmids for plasmid-vector construction and PCR analysis
- Perform plasmid sequencing and re-sequencing
- Production of high-titer viral vectors, purification and concentration of viral supernatants for retrovirus
- Production of high-titer viral vectors, purification and concentration of viral supernatants for lentivirus
- Determination of viral titer by ddPCR on fresh and frozen supernatants
- Document results of experiments and elaboration of reports for NYESO-TCR team leader
- Perform experiments associated to survival tittering and transduction efficiency on target cells
- Design and re-design of viral vectors for large scale manufacture of GMP and GMP-comparable clinical grade virus.
- Performs other job-related duties as assigned
Interested applicants may apply through this link: https://recruit.apo.ucla.edu/JPF05878
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status. For the complete University of California nondiscrimination and affirmative action policy see:
UC Nondiscrimination and Affirmative Action Policy http://policy.ucop.edu/doc/4000376/NondiscrimAffirmAct.
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