Main focus: Identification of regulators of neurodegeneration using systems biology approaches.
Mutations causing the most common form of early onset dementia, frontotemporal dementia, have been identified, but the subsequent disease mechanisms are not well understood. Recent advances in genetics have pinpointed genes causing risk for dementia in specific cell-types. This project attempts to gain important advances in understanding the basic mechanisms in neurodegeneration and find regulators of neurodegeneration. The project will employ systems biology approaches to integrate genomics, transcriptomics, proteomics and molecular biology to unravel multi-dimensional data and to find robust targets which can then be validated in in vitro and in vivo model systems of neurodegeneration
To address these challenges, several projects are required the Project Scientist oversee 1) to define distinct cell population in diseased human brain using single-nuclear transcriptomics, and develop bioinformatics approaches to find disease specific cell types; 2) to identify drivers of neurodegeneration using systems biology approaches by integrating high-dimensional data from human and mouse models; This requires implementation of CRISPR-Cas9 genomic engineering approaches to target hub or regulatory genes implicated in neurodegeneration. and 3) to study and characterize miR-203 knock-in overexpression mice.
Please list Duties in bullet points:
Direct a molecular/cell biologic/informatics research team: Design experiments, evaluate results, and plan future directions for research for the following projects.
- Define distinct cell populations in postmortem human brain samples using single-nuclear transcriptomics: • Develop approaches for transcriptional profiling of single-cells from tauopathy brains. • Use bioinformatic approaches to classify cell types based on transcriptional profiling 2) Identify drivers of neurodegeneration by using a multi-omics approach: • Use bioinformatics approaches to integrate proteomics and transcriptomics postmortem human tauopathy datasets. • Use bioinformatics approaches to integrate mouse and human data to find key drivers of the disease. 3) Develop and implement genomic engineering approaches (CRISPR/Cas9) to modulate hub genes implicated in neurodegeneration or test the function of non-coding regulatory elements in human neural progenitor cell lines. 4) Develop in vivo model systems to overexpression or downregulate hub genes involved in neurodegeneration 5) Study and characterize miR-203 overexpression mice • Characterize miR-203 knock-in mice by breeding with appropriate cre-transgenic mice. • Validate the overexpression of miR-203 and setup breeding with Tau transgenic mice. 6) Complete research and write manuscripts related to projects described for publication. 7) Assist PI with grant applications related to the research in order to gain funding for the project/personnel.
Application procedure: Apply by submitting your application to our online RECRUIT system at at https://recruit.apo.ucla.edu/apply/JPF03512 along with
CV, statement of research, and reference letters
Screening of applicants will begin immediately and will continue until the position is filled.
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